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| Carcinoma Cell Detection Kit |
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| Overview |
| The Carcinoma Cell Detection Kit allows for the rapid and highly specific immunocytochemical detection of disseminated epithelial tumor cells from human blood or bone marrow mononuclear cell cytospin preparations by intracellular staining of cytokeratins. |
| Details |
Background information
Disseminated tumor cells are detected according to their expression of epithelium-specific antigens, e.g. cytokeratins, in a non-epithelial environment, e.g. peripheral blood or bone marrow. Cytokeratins are typical intermediate filaments of the cytoskeleton of the epithelial cells1,2. Most malignant cells which have their origin in epithelial tissues express cytokeratins and can be recognized by an anti-cytokeratin antibody.
Detailed procedure
Dection of disseminated tumor cells is acheived using the Anti-Cytokeratin-Alkaline Phosphatase, a cocktail of two cytokeratin-specific antibody clones, CK3-11D5 and CK3-3E4, directly conjugated to alkaline phosphatase. Air-dried cytospin preparations are ready for microscopy within 90 minutes. |
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| Figure 1 |
| Cells of the tumor cell line SK-BR3 were transferred onto a slide by cytocentrifugation and stained using the Carcinoma Cell Detection Kit. |
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| Products |
| Carcinoma Cell Detection Kit, human |
| For research use only |
- for up to 100 tests (1)
Download datasheet
130-090-463
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| (1) One test corresponds to the analysis of 106 total cells. |
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| References |
| 1. Kasper et al. (1987) Eur. J. Cancer Clin. Oncol. 23:137-147 |
| 2. Makin, C.A. et al. (1984) J. Clin. Pathol. 37: 975-983. |
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