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| Propidium Iodide Solution |
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| Overview |
| The Propidium Iodide (PI) solution is suitable for the exclusion of dead cells from flow cytometric analysis. PI is widely used for staining and evaluation of cell death and apoptosis or for determination of DNA content in cell cycle analysis. |
| Details |
Background information
Propidium iodide (PI) is a fluorescent dye that intercalates into double-stranded nucleic acid. It is excluded from viable cells, but can penetrate cell membranes of dead or dying cells.
When excited by a 488 nm laser, PI emission can be detected in the red fluorescence channel, commonly used for PE-detection. |
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