|
|
 |
| Mouse IgM - isotype control antibodies |
|
| Overview |
| Mouse IgM isotype control antibodies are suitable for assessing the level of background staining in flow cytometric analyses of human cells. |
| Details |
Background information
The Mouse IgM isotype control antibody clone IS5-20C4 is specific for KLH (keyhole limpet hemocyanin). This protein is not expressed on human cells or cell lines. Therefore, the antibody clone IS5-20C4 can be used as a negative control to distinguish specific from non-specific binding of mouse IgM fluorochrome-conjugated antibodies to human cells, for example, via Fc receptors, or due to interactions of the fluorochrome with the cell surface. |
| Clone | Isotype |
| IS5-20C4 | Mouse IgM |
| |
| Figure 1 |
| Human PBMCs were stained with Mouse IgM antibodies conjugated to FITC (A), PE (B), APC (C) or VioGreen (D), and analyzed by flow cytometry. Cells stained with IgM-Biotin (E) were stained with Anti-Biotin-APC in addition. |
| A |
 |
| B |
 |
| C |
 |
| D |
 |
| E |
 |
|
|
|
|
|
|
© 1999 - 2012 Miltenyi Biotec ¦ Terms & conditions ¦ Legal notes
|
