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| Mouse IgG1 - isotype control antibodies |
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| Overview |
| Mouse IgG1 isotype control antibodies are suitable for assessing the level of background staining in flow cytometric analysis of human cells. |
| Details |
Background information
The Mouse IgG1 isotype control antibody clone IS5-21F5 is specific for KLH (keyhole limpet hemocyanin). This protein is not expressed on human cells or cell lines. Therefore, the antibody clone IS5-21F5 can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG1 fluorochrome-conjugated antibodies to human cells, for example via Fc receptors, or due to interactions of the fluorochrome with the cell surface. |
| Clone | Isotype |
| IS5-21F5 | Mouse IgG1 |
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| Figure 1 |
| Human PBLs were stained with Mouse IgG1 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), VioGreen (E), PerCP (F) or APC-Vio770 (G) and analyzed by flow cytometry using the MACSQuant Analyzer. Cells stained with IgG1-Biotin (H) were stained with Anti-Biotin-APC in addition. Gates were set on viable lymphocytes. |
| A |
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| B |
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| C |
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| D |
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| E |
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| F |
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| G |
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| H |
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| Products |
| Mouse IgG1-FITC |
- for 100 tests (1)
Download datasheet
130-092-213
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| Mouse IgG1-PE |
- for 100 tests (1)
Download datasheet
130-092-212
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| Mouse IgG1-APC |
- for 100 tests (1)
Download datasheet
130-092-214
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| Mouse IgG1-VioBlue |
- for 100 tests (1)
Download datasheet
130-094-670
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| Mouse IgG1-VioGreen |
- for 100 tests (1)
Download datasheet
130-096-919
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| Mouse IgG1-PerCP |
- for 100 tests (1)
Download datasheet
130-094-968
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| Mouse IgG1-APC-Vio770 |
- for 100 tests (1)
Download datasheet
130-096-653
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| Mouse IgG1-Biotin |
- for 100 tests (2)
Download datasheet
130-093-018
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| (1) One test corresponds to fluorescent labling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to labling of up to 107 cells in a total volume of 100µL. |
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