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| Overview |
| The antibody has been developed for intracellular staining of IFN-α in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by in-column intracellular staining technology in combination with MACS® Cell Separation. |
| Details |
Background information
Type I interferons (IFNs), including IFN-α, are a family of cytokines that exert pleiotropic functions in the immune system. Besides its potent antiviral activity, IFN-α exerts a variety of immunomodulatory effects, including positive and negative regulation of its own production, regulation of IL-12 and IFN-γ production, promotion of natural killer (NK) cell cytotoxicity, activation and maturation of dendritic cells, and the promotion of B cell development towards plasma cells. IFN-α is expressed predominantly by CD123+CD11c-CD303 (BDCA2)+ plasmacytoid dendritic cells (pDCs). |
| Clone | Isotype |
| LT27:295 | Mouse IgG1 |
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| Figure 1 |
| Human PBMCs were incubated with or without CpG ODN 2216 for 6 hours. After 3 hours, brefeldin A was added. Cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-α-PE. Cells were analyzed by flow cytometry. |
| A: Stimulated sample |
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| B: Unstimulated control |
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| Products |
| Anti-IFN-α-FITC, human |
- for 100 tests (1)
Download datasheet
130-092-600
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| Anti-IFN-α-PE, human |
- for 100 tests (1)
Download datasheet
130-092-601
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| Anti-IFN-α-APC, human |
- for 100 tests (1)
Download datasheet
130-092-602
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| Anti-IFN-α pure, human |
- 100 µg in 1 mL
Download datasheet
130-092-604
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
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