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| Overview |
| The Anti-GITR antibody is suitable for the identification and enumeration of human GITR+ cells by flow cytometry or fluorescence microscopy. |
| Details |
Background Information
Gluococorticoid-induced tumour necrosis factor receptor-related protein (GITR) is an inducible Type I transmembrane protein and member of the tumour necrosis factor receptor (TNFR) superfamily. GITR is also know as TNFRSF18. GITR is expressed at low levels on thymocyte subsets, resting T cells, B cells, macrophages, and at high levels on CD4+ CD25+ regulatory T Cells (Tregs). Upon activation, expression on CD4+ and CD8+ T cells is upregulated. Triggering of GITR has been described to modulate Treg function and costimulate effector T cells. Stimulation of T cells through GITR can abrogate the inhibitory function of Tregs. It is hypothesised that GITR has a role in the maintenance of immunological self tolerance, and mouse models of autoimmune disease suggest that GITR activation may break self-tolerance and induce autoimmunity. |
| Clone | Isotype |
| DT5D3 | Mouse IgG1 |
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| Figure 1 |
| Human PBMCs were stained with Anti-GITR conjugated to PE (A) or Biotin (B) as well as CD4-FITC and analyzed by flow cytometry. Cells stained with Anti-GITR-Biotin were additionally stained with Anti-Biotin-PE. |
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| B |
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| Products |
| Anti-GITR-PE, human |
- for 100 tests (1)
Download datasheet
130-092-895
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| Anti-GITR-Biotin, human |
- for 100 tests (2)
Download datasheet
130-092-886
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| Anti-GITR pure, human |
- 100 µg in 1 mL
Download datasheet
130-092-885
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 µL. |
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