Anti-Biotin-Alkaline Phosphatase ( #130-092-612 )

Anti-Biotin-Alkaline Phosphatase

Overview

The antibody is suitable for indirect labeling of frozen sections or formalin-fixed, paraffin-embedded tissues sections previously stained with a biotinylated primary antibody. It can also be used for the detection of biotinylated proteins by ELISA.

Details

Background information
MACS^® Anti-Biotin-Alkaline Phosphatase has been developed as a secondary antibody for indirect labeling in conjunction with biotinylated primary antibodies. Alkaline phosphatase works with precipitating substrates such as NBT/BCIP as well as soluble substrates such as pNPP to produce a colorimetric reaction.

Clone	Isotype
Bio3-18E7	Mouse IgG1

Figure 1

Methacarn fixed, paraffin-embedded section of a rat lymph node stained with CD45R(B220)-Biotin and Anti-Biotin Alkaline Phosphatase. Color development was achieved using NBT/BCIP as a substrate.