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| Overview |
The antibody is suitable for identification and enumeration of CD158b (KIR2DL2/DL3)+ cells by flow cytometry and fluorescence microscopy.
The family of killer-immunoglobulin-like receptors (KIR) contributes to theregulation of NK cell-mediated cytotoxicity. |
| Details |
Background information
Clone DX27 recognizes CD158b1 (KIR2DL2) and CD158b2 (KIR2DL3), members of the killer immunoglobulin-like receptor (KIR) family expressed on natural killer (NK) cells and subsets of T cells.
KIRs contribute to the regulation of NK cell–mediated cytotoxicity and possess high allelic polymorphism with either 2 or 3 Ig-like extracellular domains. According to the length of their cytoplasmic tail, KIRs can be further subdivided in inhibitory KIRs (KIR2DL or KIR3DL) and activating KIRs (KIR2DS or KIR3DS). CD158b provides an inhibitory signal on NK cell lytic activity upon interaction with HLA C (e.g. alleles HLA-Cw1, HLA-Cw3, HLA-Cw7) in an antigen-independent manner. |
| Clone | Isotype |
| DX27 | Mouse IgG2a |
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| Figure 1 |
| Human PBMCs were stained with CD158b (KIR2DL2/DL3)-PE (A), -APC (B), or -PerCP (C) and analyzed by flow cytometry using the MACSQuant® Analyzer. |
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| B |
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| C |
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| Products |
| CD158b (KIR2DL2/DL3)-PE, human |
- for 100 tests (1)
Download datasheet
130-092-618
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| CD158b (KIR2DL2/DL3)-APC, human |
- for 100 tests (1)
Download datasheet
130-092-617
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| CD158b (KIR2DL2/DL3)-PerCP, human |
- for 100 tests (1)
Download datasheet
130-095-285
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| CD158b (KIR2DL2/DL3) pure, human |
- 100 µg in 1 mL
Download datasheet
130-092-615
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 µL. |
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