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| Overview |
| The antibody is suited for the identification and enumeration of mouse GITR+ cells by flow cytometry. In vitro, the Anti-GITR antibody abrogates Treg cell–mediated supression. |
| Details |
Background information
Clone DTA-1 specifically recognizes mouse GITR (Glucocorticoid-induced TNF-receptor), a member of the TNF receptor superfamily also known as TNFRSF18. It is expressed at low levels on resting T lymphocytes and at high levels on CD4+CD25+ regulatory T cells (Tregs). Activation of T cells upregulates GITR expression. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production, as well as MAPKs and NF-κB activation. GITR plays an important role in the function of CD4+CD25+ Tregs. |
| Clone | Isotype |
| DTA-1 | Rat IgG2b |
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| Figure 1 |
| CD4+ mouse splenocytes were stained with Anti-GITR-PE (A) or Anti-GITR-APC (B) and CD25-Biotin/Anti-Biotin-APC or CD25-PE. Cells were analyzed by flow cytometry. |
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| B |
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| Products |
| Anti-GITR-PE, mouse |
- for 100 tests (1)
Download datasheet
130-092-469
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| Anti-GITR-APC, mouse |
- for 100 tests (1)
Download datasheet
130-092-470
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| Anti-GITR pure, mouse |
- 100 µg in 1 mL
Download datasheet
130-092-630
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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