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| Mouse IgG2b - isotype control antibodies |
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| Overview |
| Mouse IgG2b isotype control antibodies are suitable for assessing the level of background staining in flow cytometric analyses of human cells. |
| Details |
Background information
The Mouse IgG2b isotype control antibody clone IS6-11E5.11 is specific for KLH (keyhole limpet hemocyanin). This protein is not expressed on human cells or cell lines. Therefore, the antibody clone IS6-11E5.11 can be used as a negative control, to distinguish specific from non-specific binding of mouse IgG2b fluorochrome-conjugated antibodies to human cells, for example, via Fc receptors, or due to interactions of the fluorochrome with the cell surface. |
| Clone | Isotype |
| IS6-11E5.11 | Mouse IgG2b |
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| Figure 1 |
| Human PBMCs were stained with Mouse IgG2b-FITC (A), Mouse IgG2b-PE (B), Mouse IgG2b-APC (C), Mouse IgG2b-VioGreen (D) or Mouse IgG2b APC-Vio770 (E) and analyzed by flow cytometry. |
| A |
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| B |
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| C |
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| D |
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| E |
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