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| Overview |
| The VIT4 antibody clone can be used for the identification and enumeration of human CD4+ cells by flow cytometry or fluorescence microscopy. The CD4 antibody recognizes most thymocytes and about 65% of peripheral blood T cells. It is suitable for staining of fixed cells. |
| Details |
Background information
The CD4 antibody recognizes the human CD4 antigen which is highly expressed on human T helper cells and thymocytes, and at lower levels on monocytes and dendritic cells. It is responsible for the recognition of the MHC Class II antigen.
The CD4 antibodies can be used for evaluating the transfection efficiency of cells transfected with pMACS 4.1 or pMACS 4-IRES when using the MACSelect™ 4 Transfected Cell Selection Kit. |
| Clone | Isotype |
| VIT4 | Mouse IgG2a |
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| Figure 1 |
| Human PBMCs were stained with CD4 (VIT4) antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), VioGreen (E), PerCP (F), PE-Vio770 (G), or APC-Vio770 (H) and analyzed by flow cytometry. |
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| B |
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| C |
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| D |
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| E |
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| F |
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| G |
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| H |
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