CD69 ( #130-092-166 #130-092-160 #130-092-159 #130-092-161 )

CD69

Overview

The CD69 antibody can be used to identify and enumerate human CD69⁺ cells by flow cytometry or fluorescence microscopy. It is suitable for studies of T cell activation and for analysis of cytokine expression in combination with cytokine secretion assays or intracellular cytokine staining.

Details

Background information
Clone FN50 recognizes the human CD69 antigen, also known as activation inducer molecule (AIM). CD69 is involved in the early events of lymphocyte, monocyte and platelet activation. Cross-linking of CD69 induces cytotoxic activity and costimulates cytokine production of activated NK cells and selected T cell clones. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. It is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4⁺ T cells in germinal centers of normal lymph nodes.

Cross-reactivity

The antibody is tested to cross-react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Furthermore, the antibody is reported to cross-react with PBLs from baboon^1.

Clone	Isotype
FN50	Mouse IgG1

Figure 1

Human peripheral blood leukocytes were stained with CD69 antibodies conjugated to FITC (A), PE (B), APC (C) or Biotin (D). Cells were counterstained with CD4 antibodies and analyzed by flow cytometry.

Figure 2

Rhesus monkey (A) or cynomolgus monkey (B) PBMCs were stimulated with Anti-Biotin MACSiBead™Particles loaded with CD2, CD3, and CD28 antibodies. Cells were stained with CD69-PE and CD4-APC and analyzed by flow cytometry.