CD45RA ( #130-092-247 #130-092-248 #130-092-249 #130-095-464 #130-095-466 #130-096-259 #130-096-604 #130-096-921 )

CD45RA

Overview

The antibody is suited for the identification and enumeration of human and non-human primate CD45RA⁺ cells by flow cytometry or fluorescence microscopy.

Details

Background information
Clone T6D11 recognizes the human CD45RA antigen which is expressed on naive CD4⁺ and CD8⁺ T cells as well as on CD8⁺ effector T cells. CD45RA is also present on subsets of B and NK cells as well as on plasmacytoid dendritic cells. The CD45RA antibody recognizes the 220 kDa isoform of the leukocyte common antigen (LCA), a transmembrane tyrosine phosphatase.

Cross-reactivity

This antibody has been tested to cross-react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Clone	Isotype
T6D11	Mouse IgG2b

Figure 1

Human PBMCs were stained with CD45RA antibodies coupled to FITC (A), PE (B), APC (C), VioBlue (D), PerCP (E) or VioGreen (F) and CD45RO-PE or -FITC, and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD45RA-Biotin were stained with Anti-Biotin-PE (G) as well as CD45RO-APC.

Figure 2

Rhesus monkey PBMCs were stained with CD45RA-PE and CD4-FITC and analyzed by flow cytometry.

Figure 3

Cynomolgus monkey PBMCs were stained with CD45RA-PE and CD3-APC and analyzed by flow cytometry.