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 - Proven and tested: tool of choice in more than 500 publications
- Exclusive: developed by Miltenyi Biotec, available from Miltenyi Biotec only
- Unique: CD133 antibodies for analysis of primitive stem cells
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| Overview |
The CD133/1 antibodies allow for the identification and enumeration of CD133+ cells by flow cytometry, e.g.,
- hematopoietic stem cells from peripheral blood, cord blood, bone marrow, or leukapheresis product,
- neural progenitor cells from single-cell suspensions from primary neural tissues or cell lines,
- ES and iPS cell derived neural, endothelial, or hematopoietic progenitors from differentiated ES or iPS cell cultures1,
- cancer stem cells from single-cell suspensions from primary tumor tissue or cell lines.
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| Details |
Background information
CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen.2,3 It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34– cells4 as well as on a subset of CD34bright stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood5. CD133 has also been found to be expressed on circulating endothelial progenitor cells6,7, fetal neural stem cells8,9, other tissue-specific stem cells, such as renal10, prostate11, and corneal12 stem cells, cancer stem cells from tumor tissues, as well as ES and iPS cell-derived cells. |
| Clone | Isotype |
| AC133 | mouse IgG1 |
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| Figure 1 |
| Staining of non-mobilized PBMCs with CD133/1-PE and CD45-FITC. |
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| Figure 2 |
| Staining of non-mobilized PBMCs with CD133/1-APC and CD34-FITC. |
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| Products |
| CD133/1 (AC133)-PE, human |
- for 100 tests (1)
Download datasheet
130-080-801
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| CD133/1 (AC133)-APC, human |
- for 100 tests (1)
Download datasheet
130-090-826
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| CD133/1 (AC133)-Biotin, human |
- for 100 tests (2)
Download datasheet
130-090-664
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| CD133/1 (AC133) pure, human |
- 50 μg in 1 mL
Download datasheet
130-090-422
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Galic et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103: 11742–11747 |
| 2. Yin, A. H. et al. (1997) Blood 90: 5002–5012 |
| 3. Piechaczek, C. (2001) J Biol Reg Hom Agents 15: 101–102. |
| 4. Gallacher, L. et al. (2000) Blood 95: 2813–2820. |
| 5. Bühring, H. J. et al. (1999) Ann NY Acad Sci 872: 25–39. |
| 6. Gehling, U. M. et al. (2000) Blood 95: 3106–3112. |
| 7. Peichev, M. et al. (2000) Blood 95: 952–958. |
| 8. Uchida et al. (2000) Proc. Natl. Acad. Sci. USA 97: 14720–14725. |
| 9. Cummings et al. (2005) Proc. Natl. Acad. Sci. USA 102: 14069–14074. |
| 10. Bussolati, B. et al. (2005) Am. J. Pathol. 166: 545–555. |
| 11. Richardson, G. et al. (2004) J. Cell Sci. 117: 3539–3545. |
| 12. Thill, M. et al. (2004) Invest. Opthalmol. Vis. Sci. 45: 3519. |
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