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| Overview |
| The CD146 antibody is used to identify and enumerate mouse endothelial cells, including liver sinusoidal endothelial cells (LSECs) or other CD146+ cells by flow cytometry or fluorescence microscopy. |
| Details |
Background information
The CD146 (LSEC) antibody (clone ME-9F1) binds to the CD146 antigen, which is expressed on mouse endothelial cells, including liver sinusoidal endothelial cells (LSECs), smooth muscle cells, and the basal membrane.1
LSECs are microvascular endothelial cells lining the hepatic sinusoidal wall. Their strategic positioning favors a tight interaction with lymphocytes migrating through the liver. They possess a high capacity for antigen uptake and processing. However, in contrast to professional antigen-presenting cells (e.g. dendritic cells), they express only low levels of costimulatory molecules.2 |
| Clone | Isotype |
| ME-9F1 | Rat IgG2a |
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| Figure 1 |
| Mouse liver cells were stained with CD146 (LSEC) antibodies conjugated to FITC (A) or Biotin (B). Cells stained with CD146 (LSEC)-Biotin were also stained with Anti-Biotin-APC and analyzed by flow cytometry. |
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| B |
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| Products |
| CD146 (LSEC)-FITC, mouse |
- for 100 tests (1)
Download datasheet
130-092-026
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| CD146 (LSEC)-Biotin, mouse |
- for 100 tests (2)
Download datasheet
130-092-025
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Harder et al. (1991) Exp. Cell Res. 197: 259–267. |
| 2. Diehl et al. (2008) Hepatology 47: 296–305. |
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