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| Overview |
| The monoclonal CD117 antibody A3C6E2 recognizes the human SCF receptor (c-kit) and is ideal for the detection and quantification of CD117-expressing cells by flow cytometry or immunofluorescence1. |
| Details |
Background information
CD117 (also known as c-kit or SCF receptor) is a 145 kDa receptor tyrosine kinase that is involved in stem cell signaling, activation, and proliferation. The CD117 antigen is expressed on about 1-3% of mononuclear cells from peripheral blood (PBMCs) or cord blood, and on up to 10% of bone marrow cells. CD117 is further expressed on basophils, myeloid dendritic cells, TCRα/β + T cells, CD19+ B cells, and CD56+ NK cells2 , as well as on mast cells, melanocytes, and AML (acute myeloid leukemia) blasts3.
Clone A3C6E2 recognizes a different epitope than clone AC126 and may interfere with SCF-binding. It is therefore not recommended for indirect cell isolation, but is optimal for flow cytometric analysis of cells separated with the CD117 MicroBead Kit, human. |
| Clone | Isotype |
| A3C6E2 | Mouse IgG1 |
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| Figure 1 |
| Flow cytometric analysis of human cord blood mononuclear cells (CB-MNCs) with CD117-PE (A) or -APC (B) and CD133 antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and PI fluorescence. |
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| B |
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| Products |
| CD117 (A3C6E2)-PE, human |
- for 100 tests (1)
Download datasheet
130-091-734
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| CD117 (A3C6E2)-APC, human |
- for 100 tests (1)
Download datasheet
130-091-733
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Kubo et al. (2008) Circulation 118: 649-657. |
| 2. Guth et al. (1995) (Abstract) 9th International Congress of Immunology. |
| 3. Bühring et al. (1991) Leukemia 5: 854–860. |
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