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| Mouse IgG2a - isotype control antibodies |
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| Overview |
| Mouse IgG2a isotype control antibodies are suitable for assessing the level of background staining in flow cytometric analysis of human cells. |
| Details |
Background information
The Mouse IgG2a isotype control antibody clone S43.10 is specific for the hapten NP (4-hydroxy-3-nitro-phenyl) acetyl. This hapten is not expressed on cells or cell lines. Therefore, the antibody clone S43.10 can be used as a negative control to distinguish specific from non-specific binding of mouse IgG2a fluorochrome-conjugated antibodies to human cells, for example, via Fc receptors or due to interactions of the fluorochrome with the cell surface. |
| Clone | Isotype |
| S43.10 | Mouse IgG2a |
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| Figure 1 |
| Human peripheral blood lymphocytes were stained with Mouse IgG2a antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), VioGreen (E), PerCP (F), PE-Vio770 (G) or APC-Vio770 (H) and analyzed by flow cytometry. Gates were set on viable lymphocytes. |
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| B |
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| C |
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| D |
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| E |
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| F |
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| G |
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| H |
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