Anti-Cytokeratin-Alkaline Phosphatase ( #130-090-462 )

Anti-Cytokeratin-Alkaline Phosphatase

Overview

Anti-Cytokeratin-Alkaline Phosphatase is suitable for immunohistochemistry. It is used for intracellular staining of epithelial tissue and detection of epithelium-derived tumor cells in non-epithelial cell samples or tissues.

Details

Background information
Anti-Cytokeratin-Alkaline Phosphatase is a cocktail of two cytokeratin-specific antibody clones, CK3-11D5 and CK3-3E4, which are directly conjugated to alkaline phosphatase. CK3-11D5 is a multi-cytokeratin–specific antibody that recognizes cytokeratins 7, 8, 18, and 19 and crossblocks binding of Cam5.2, an antibody specific for cytokeratins 7 and 8. CK3-3E4 binds specifically to cytokeratin 8 and almost completely crossblocks A45-B/B3, an antibody known to recognize the cytokeratin heterodimers 8/18 and 8/19.

Clone	Isotype
CK3-3E4	Mouse IgG2a
CK3-11D5	Mouse IgG1

Figure 1

Cells of the breast cancer cell line SK-BR-3 were spiked into PBMCs of a healthy donor and subjected to intracellular staining by using the solid-phase intracellular staining technology in combination with MACS^® Separation and Anti-Cytokeratin-Alkaline Phosphatase.