Anti-IFN-γ ( #130-091-641 #130-091-653 #130-091-640 #130-096-625 #130-096-752 )

Anti-IFN-γ

Overview

This Anti-IFN-γ antibody is suitable for intracellular staining of IFN-γ in fixed and permeabilized human and non-human primate cells for flow cytometric analysis. Cells can be stained in suspension or by in-column intracellular staining technology in combination with MACS® Separation. For functional assays such as neutralization of IFN-γ activity use the functional grade antibody.

Details

Background information
Interferon gamma (IFN-γ) is produced by cells that are involved in inflammatory immune responses. IFN-γ is predominantly released by memory and effector CD4⁺ and CD8⁺ T cells as well as by NK cells upon activation.

Cross-reactivity

The antibody is tested to cross-react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Clone	Isotype
45-15	Mouse IgG1

Figure 1

Human PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ-PE. Cells were then analyzed by flow cytometry. Plots show CD4⁺ lymphocytes gated according to CD4-FITC staining.

Stimulated sample

Unstimulated control

Figure 2

Rhesus monkey PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ-PE. Cells were analyzed by flow cytometry. Plots show CD4+ lymphocytes gated according to CD4-APC staining.

Stimulated sample

Unstimulated control

Figure 3

Cynomolgus monkey PBMCs were incubated with or without SEB for six hours. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IFN-γ-PE. Cells were analyzed by flow cytometry.

Stimulated sample

Unstimulated control