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| Overview |
| The antibody can be used to identify and enumerate mouse Gr-1+ cells, for example mature granulocytes, by flow cytometry or fluorescence microscopy. Anti-Gr-1-Biotin can also be used for depletion of granulocytes/monocytes in combination with Anti-Biotin MicroBeads. |
| Details |
Background information
The mouse Anti-Gr-1 antibody reacts with Ly-6G, a 21-25 kDa, GPI-anchored cell surface protein, previously defined as the granulocyte-differentiation antigen-1 (Gr-1).1 The antibody has been shown to cross-react weakly with Ly-6C-transfected EL-4J cells.2 Cross-reactivity of Anti-Gr-1 with Ly-6C was not detected on hematopoietic cells that express Ly-6C and are negative for Ly-6G.3
Gr-1 is expressed on mature granulocytes in bone marrow and peripheral tissues. The Anti-Gr-1 antibody also stains monocytes transiently during their differentiation in bone marrow and at low levels plasmacytoid dendritic cells in lymphoid tissues. |
| Clone | Isotype |
| RB6-8C5 | Rat IgG2b |
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| Figure 1 |
| Mouse spleen cells were stained with Anti-Gr-1 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), PerCP (E) or Biotin (F) and CD45R (B220) and analyzed by flow cytometry. Cells stained with Anti-Gr-1-Biotin were stained with Anti-Biotin-APC in addition. |
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| Products |
| Anti-Gr-1-FITC, mouse |
- for 100 tests (1)
Download datasheet
130-091-934
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| Anti-Gr-1-PE, mouse |
- for 100 tests (1)
Download datasheet
130-091-932
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| Anti-Gr-1-APC, mouse |
- for 100 tests (1)
Download datasheet
130-091-931
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| Anti-Gr-1-VioBlue, mouse |
- for 100 tests (1)
Download datasheet
130-094-362
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| Anti-Gr-1-PerCP, mouse |
- for 100 tests (1)
Download datasheet
130-094-958
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| Anti-Gr-1-Biotin, mouse |
- for 100 tests (2)
Download datasheet
130-091-930
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Hestdal et al. (1991) J. Immunol. 147: 22–28. |
| 2. Fleming et al. (1993) J. Immunol. 151: 2399–2408. |
| 3. Nagendra et al. (2004) Cytometry A 58: 195–200. |
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