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| Overview |
| The CD90.2 antibody can be used to identify and enumerate mouse CD90+ cells by flow cytometry or fluorescence microscopy. The CD90.2 antibody clone 30-H12 reacts with the Thy1.2 alloantigen, which is a pan–T cell marker for the most common inbred mouse strain. These strains include BALB/c, DBA, CBA/J, C3H, C57BL/6, NZB/-, S3L and others. It does not cross-react with Thy1.1. |
| Details |
Background information
Clone 30-H12 recognizes the mouse CD90 (Thy1.2) antigen which is expressed at high levels on thymocytes and peripheral T cells in lymphoid organs and blood as well as on retinal ganglion cells. CD90 is also expressed at lower levels on early hematopoietic stem cells in the bone marrow, on interepithelial cells (dendritic epidermal T cells) in skin, and on neurons. |
| Clone | Isotype |
| 30-H12 | Rat IgG2b |
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| Figure 1 |
| Mouse splenocytes were stained with CD90.2 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PerCP (E) and analyzed by flow cytometry using the MACSQuant® Analyzer. |
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| B |
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| C |
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| D |
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| E |
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