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| Overview |
| The CD45R (B220) antibody can be used to identify and enumerate mouse CD45R (B220)+ cells by flow cytometry or fluorescence microscopy. |
| Description |
Background information
Clone RA3-6B2 recognizes the mouse CD45R (B220) antigen which is expressed on B lymphocytes throughout their development from early pro-B stages onwards and is down-regulated upon terminal differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R monoclonal antibody clone RA3-6B2 specifically recognizes the exon A–restricted isoform of mouse CD45. CD45R is absent in thymus but reported to be present on apoptotic thymocytes. |
| Clone | Isotype |
| RA3-6B2 | Rat IgG2a |
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| Figure 1 |
| Mouse splenocytes were stained with CD45R (B220) antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), VioGreen (E), PerCP (F), PE-Vio770 (G), or APC-Vio770 (H) and analyzed by flow cytometry using the MACSQuant® Analyzer. |
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| B |
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| C |
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| D |
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| E |
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| F |
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| G |
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| H |
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