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| Overview |
| The CD45 antibody is suitable to identify and enumerate mouse CD45+ cells by flow cytometry or fluorescence microscopy. CD45 (Ly-5, leukocyte common antigen) can be used to discriminate leukocytes from nonhematopoietic cells. |
| Details |
Background information
Clone 30F11 recognizes the mouse CD45 antigen (Ly-5, leukocyte common antigen) which is expressed at high levels on all cells of hematopoietic origin except for erythrocytes. The CD45 antibody clone 30F11 reacts with all CD45 isoforms. |
| Clone | Isotype |
| 30F11 | Rat IgG2b |
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| Figure 1 |
| Mouse splenocytes were stained with CD45 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), or PerCP (D) and analyzed by flow cytometry using the MACSQuant® Analyzer.. |
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| B |
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| C |
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| D |
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| E |
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