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| Overview |
| The CD8a antibody can be used to identify and enumerate mouse CD8a+ cells by flow cytometry or fluorescence microscopy. CD8a is present on approximately 10% of splenocytes, 25% of lymph node cells, and 85% of thymocytes in healthy mice. |
| Applications |
Background information
Clone 53-6.7 is specific for the mouse CD8a (Ly-2) antigen, which is expressed on cytotoxic T cells and at lower levels on subpopulations of dendritic cells and TCRγ/δ+ cells. It is further detected on most thymocytes (CD4+CD8a+ and CD4–CD8+ thymocytes). Cytotoxic T cells and thymocytes express CD8a as heterodimer with CD8b, whereas dendritic cells and TCRγ/δ+ cells express a CD8a/CD8a homodimer. |
| Clone | Isotype |
| 53-6.7 | Rat IgG2a |
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| Figure 1 |
| Mouse splenocytes were stained with CD8a antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), VioGreen (E), PerCP (F), PE-Vio770 (G), or APC-Vio770 (H) and analyzed by flow cytometry using the MACSQuant® Analyzer. |
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| B |
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| D |
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| E |
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| F |
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| G |
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| H |
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