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| Overview |
| The CD4 antibody can be used to identify and enumerate mouse CD4+ cells by flow cytometry or fluorescence microscopy. CD4 is present on approximately 25% of splenocytes, 55% of lymph node cells, and 90% of thymocytes in naive mice. |
| Details |
Background information
Clone GK1.5 recognizes the mouse CD4 (L3T4) antigen. In mice, CD4 is expressed on T helper cells, regulatory T cells, and at lower levels on subpopulations of NKT cells and dendritic cells. It is furthermore detected on most thymocytes (CD4+CD8+ and CD4+CD8– thymocytes). |
| Clone | Isotype |
| GK1.5 | Rat IgG2b |
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| Figure 1 |
| Mouse splenocytes were stained with CD4 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), APC-Vio770 (E) or VioGreen (F), and analyzed by flow cytometry. |
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| B |
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| D |
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| E |
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| F |
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| Products |
| CD4-FITC, mouse |
- for 100 tests (1)
Download datasheet
130-091-608
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| CD4-PE, mouse |
- for 100 tests (1)
Download datasheet
130-091-607
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| CD4-APC, mouse |
- for 100 tests (1)
Download datasheet
130-091-611
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| CD4-VioBlue, mouse |
- for 100 tests (1)
Download datasheet
130-096-056
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| CD4-VioGreen, mouse |
- for 100 tests (2)
Download datasheet
130-096-898
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| CD4-APC-Vio770, mouse |
- for 100 tests (1)
Download datasheet
130-096-608
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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