Anti-mPDCA-1

Unique antibody for DC research
Accurate PDC detection with just a single marker

Overview

Anti-mPDCA-1 antibodies allow the direct, single-color detection of mouse plasmacytoid dendritic cells (PDCs) by flow cytometry, fluorescence microscopy, or immunohistochemistry. They are an excellent tool for the functional analysis of PDCs directly isolated from tissues, in vitro-generated from bone marrow cell cultures, or by in vivo depletion. Furthermore, Anti-mPDCA-1 antibodies are suitable for studies on PDCs in experimental mouse models of infection or autoimmune disease regarding tissue distribution, frequency, specific localization within lymphoid organs, and recruitment to inflamed tissues. The antibodies are also recommended for evaluation of the purity of mouse PDCs isolated with the Plasmacytoid Dendritic Cell Isolation Kit II, Anti-mPDCA-1 MicroBeads or CD11c MicroBeads.

Details

Background information
Clone JF05-1C2.4.1 specifically recognizes the mouse plasmacytoid dendritic cell antigen-1 (mPDCA-1). mPDCA-1 is specifically expressed on mouse PDCs — a subset of CD11c⁺ dendritic cells detected at low frequency in all lymphoid tissues, peripheral blood, and some non-lymphoid tissues.
In mouse spleen, bone marrow, and lymph nodes, mPDCA-1 is exclusively expressed on cells which are CD11c^low, Ly-6C^high, CD45R (B220)⁺, MHC Class II^low, CD8a^low/–, CD80^low/–, CD86^–, CD40^–, CD11b^–, CD90^–, CD49b (DX5)^–, CD3^–, CD19^–, i.e. on cells with the phenotype of mouse PDCs. Multi-color fluorescent staining of spleen cells clearly revealed that all CD11c^low Ly-6C^high CD45R (B220)⁺ PDCs are mPDCA-1⁺ and that mPDCA-1 expression is restricted to PDCs.

Clone	Isotype
JF05-1C2.4.1	Rat IgG2b

Figure 1

Identification of CD11c⁺ Ly-6C⁺ CD45R (B220)⁺ PDCs with Anti-mPDCA-1.
Splenocytes isolated from BALB/c mice were analyzed by multi-color fluorescent staining with CD11c, Anti-Ly-6C, CD45R (B220), and Anti-mPDCA-1. Cells expressing Ly-6C^high CD45R (B220)^high (A) are also positive for CD11c and mPDCA-1 (B).
Note that all CD11c^low Ly-6C^high CD45R (B220)⁺ PDCs are mPDCA-1⁺ and that mPDCA-1 expression is restricted to PDCs only.

Figure 2

Direct identification of PDCs with Anti-mPDCA-1-FITC. Splenocytes were stained with Anti-PDCA-1-FITC and Anti-Ly-6C-PE. Note that staining with Anti-mPDCA-1-FITC alone is sufficient for direct detection of murine PDCs.