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| Overview |
| The CD16 antibody can be used to identify and enumerate human and non-human primate CD16+ cells by flow cytometry or fluorescence microscopy. |
| Details |
Background information
CD16 is a 50–80 kDa glycoprotein that is expressed in two different isoforms. The transmembrane form is found on human NK cells, macrophages, and mast cells, while the glycosylphosphatidylinositol (GPI)-linked form is present on neutrophils.
The human CD16 antigen is a low-affinity receptor for aggregated IgG. The transmembrane form plays a role in signal transduction, NK cell activation, and antibody-dependent cellular cytotoxicity. Clone VEP13 recognizes both the extracellular domain of the transmembrane form as well as the GPI-linked form of the human CD16 antigen. CD16 is expressed on the majority of rhesus monkey NK cells and on a subset of monocytes but not on granulocytes. |
| Cross-reactivity |
This antibody is tested to cross-react with - Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| VEP13 | Mouse IgM |
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| Figure 1 |
| Human lysed blood was stained with CD16-FITC (A), CD16-PE (B), and CD16-APC (C), CD16-VioGreen (D) or CD16-APC-Vio770 (E) and analyzed by flow cytometry. |
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| B |
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| D |
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| E |
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| Figure 2 |
| Rhesus monkey PBMCs were stained with CD16-PE and analyzed by flow cytometry. |
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| Figure 3 |
| Cynomolgus monkey PBMCs were stained with CD16-FITC and analyzed by flow cytometry. |
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