Fixation and Dead Cell Discrimination Kit ( #130-091-163 )

Fixation and Dead Cell Discrimination Kit

Overview

The Fixation and Dead Cell Discrimination Kit allows fixation of cells and exclusion of dead cells by flow cytometry. The kit is suitable for staining of nucleated dead cells of any species.

Details

The Fixation and Dead Cell Discrimination Kit allows dead cell staining to be combined with cell fixation. The kit contains reagents for fixation and dead cell fluorescent staining. Fixation of cells facilitates working with biohazardous samples and also allows the prolonged storage of samples. Before fixation, dead cells are labeled irreversibly with a membrane-impermeable dye that binds covalently to nucleic acid upon exposure to visible light. This staining remains stable after fixation meaning that cells which were dead prior to fixation can be excluded by flow cytometric analysis. Thus, non-specific background staining is significantly reduced and sensitivity of detection enhanced.
The Fixation and Dead Cell Discrimination Kit can be used for:
• Prolonged storage of cell samples
(up to 24 hours)
• Reduced biohazard of infectious samples due to fixation of the cells
• Highly sensitive analysis of rare cells
due to the reduction of background staining caused by dead cells

Figure 1

Principle of the Fixation and Dead Cell Discrimination Kit.
Fixation reduces sample hazard and allows storage for up to 24 hours prior to flow cytometric acquisition. Sensitivity of analysis is significantly enhanced due to discrimination of dead cells from viable.