Inside Stain Kit ( #130-090-477 )

Inside Stain Kit

Overview

The Inside Stain Kit contains reagents for the fixation and permeabilization of cells for intracellular staining, for example for flow cytometric evaluation. The kit was also optimized to function in combination with MACS^® Technology.
Typical applications of the Inside Stain Kit include:

Intracellular staining of cytokine producing cells for cytokine detection by flow cytometry
Intracellular staining of enriched cells, e.g., CD138⁺ cells for detection of intracellular kappa/lambda light chains, or melanoma cells for detection of Melan A expression
Intracellular staining of other cells enriched using MACS Technology

Details

Background information
The Inside Stain Kit and the newly developed protocol for solid-phase intracellular staining allow the fast, easy, and efficient staining of cells while they are immobilized within a MACS Column during separation. Thus, extra centrifugation steps are unnecessary, minimizing cell loss. Typically, separated cells eluted from the first column are fixed in suspension by adding Inside Fix. After direct application of the cells to a second column, permeabilization and staining reagents are added. The column is then removed from the magnetic field and the stained cells are eluted and directly analyzed. This solid-phase, intracellular staining requires only very small volumes of staining reagents. Optimal staining in combination with MACS Technology can be achieved on MS Columns.

Columns

Optimal staining can of enriched cells using MACS Technology be achieved using MS Columns.

Figure 1

CD138⁺ plasma cells isolated from PBMCs of a healthy human donor were stained with Anti-kappa-light-chain-FITC, CD138-PE, and Anti-lambda-light-chain-Cy®5 using the Inside Stain Kit by solid-phase intracellular staining in combination with MACS Technology.

A: Gating on CD138⁺ cells

B: Staining of light chains

Figure 2

Solid-phase intracellular staining technology in combination with MACS Technology.