Anti-Biotin ( #130-090-857 #130-090-756 #130-090-856 #130-094-669 #130-094-974 #130-096-630 #130-097-022 #130-096-632 )

Anti-Biotin

Overview

The Anti-Biotin fluorochromes are perfectly suited for indirect immunofluorescent labeling when working with a biotinylated primary antibody. They can also be used for the simultaneous staining of several cell surface markers when working with a cocktail of biotinylated antibodies. The Anti-Biotin antibody does not bind to free biotin which is often present in cell culture media.

Details

Background information
Anti-Biotin fluorochromes are ideal for fluorescent staining of cells separated with Anti-Biotin MicroBeads, Streptavidin MicroBeads, or with the Anti-Biotin MultiSort Kit. They can also be used to stain cells labeled with cocktails of biotinylated antibodies, for example for quality control when using MACS Cell Isolation Kits for the isolation of untoched cells.

Clone	Isotype
Bio3-18E7	Mouse IgG1

Figure 1

Human PBMCs were labeled with CD303 (BDCA-2)-Biotin and fluorescently stained with Anti-Biotin antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), PerCP (E), VioGreen (F), PE-Vio770 (G), or APC-Vio770 (H). Cells were counterstained with CD304 (BDCA-4/Neuropilin-1)-PE or -APC, respectively, and analyzed by flow cytometry using the MACSQuant Analyzer^®.