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| Overview |
| The CD25 antibody is suitable for identification and enumeration of mouse CD25+ cells, e.g. activated T and B cells. In combination with CD4, it can be used to identify regulatory T cells (Tregs). |
| Details |
Background information
The CD25 antibody recognizes the α chain of the IL-2 receptor (IL-2R). IL-2Rα associates with the β chain (CD122) and the γ chain (CD132) to form the functional IL-2R complex. IL-2Rα is expressed on CD4+CD25+ regulatory T cells, activated T and B cells, and to a lesser extent on activated dendritic cells. It is also transiently expressed during T and B cell development. Binding of the CD25 antibody does not impede the binding of IL-2 to its receptor. |
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| Figure 1 |
| Mouse splenocytes were stained with CD25-PE (A) or CD25-Biotin and Anti-Biotin-PE (B) and CD4-FITC and analyzed by flow cytometry. |
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| B |
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| Products |
| CD25-PE, mouse |
- for 100 tests (1)
Download datasheet
130-091-013
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| CD25-APC, mouse |
- for 100 tests (1)
Download datasheet
130-093-734
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| CD25-Biotin, mouse |
- for 100 tests (2)
Download datasheet
130-092-569
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
| (2) One tests corresponds to labeling of up to 107 cell in a total volume of 100 µL. |
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