CD25 ( #130-091-024 #130-091-235 #130-092-858 )

CD25

Overview

The CD25 antibody of the clone 4E3 can be used to identify and enumerate human and non-human primate cells by flow cytometry or fluorescence microscopy. This antibody is highly suitable for MACS^® Control applications after using CD25 MicroBeads. The CD25 antibody recognizes a different epitope (epitope B) than the CD25 MicroBeads (epitope A). For further details, please refer to the data sheet of the separation reagent.

Details

Background information
The CD25 antibody recognizes the human CD25 antigen, a 55 kDa glycoprotein also known as the low-affinity interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed on activated T and B cells, on macrophages, and on a subset of non-activated CD4⁺ regulatory T cells.
The CD25 antigen contains three epitope regions called A, B, and C. This CD25 antibody recognizes epitope region B.

Cross-reactivity

This antibody is tested to cross-react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Clone	Isotype
4E3	Mouse IgG2b

Figure 1

CD4⁺ human cord blood lymphocytes were isolated with the CD4⁺ T Cell Isolation Kit II, stained with CD25-PE, and analyzed by flow cytometry.

CD4<sup>+</sup> human cord blood lymphocytes were isolated with the <a href="/en/PG_327_13_CD4_T_Cell_Isolation_Kit_II.aspx">CD4<sup>+</sup> T Cell Isolation Kit II</a>, stained with CD25-PE, and analyzed by flow cytometry.

Figure 2

Rhesus monkey PBMCs were stained with CD25-PE and CD4-APC and analyzed by flow cytometry.

Figure 3

Cynomolgus monkey peripheral blood cells were stained with CD25-PE and CD4-APC and analyzed by flow cytometry.
(Data were kindly provided by the California National Primate Research Center, Davis, CA, USA.)

Cynomolgus monkey peripheral blood cells were stained with CD25-PE and CD4-APC and analyzed by flow cytometry. <br>(Data were kindly provided by the California National Primate Research Center, Davis, CA, USA.)