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| Overview |
| The CD14 antibody can be used to identify and enumerate CD14+ cells by flow cytometry or fluorescence microscopy. CD14 is a high-affinity receptor for the complex of lipopolysaccharide (LPS) and LPS-binding protein (LBP). |
| Details |
Background information
Clone TÜK4 recognizes the human CD14 antigen and cross-reacts with non-human primate CD14. The CD14 antigen is part of the functional heteromeric LPS receptor complex comprised of CD14, TLR4, and MD-2. CD14 is strongly expressed on most human monocytes and macrophages in peripheral blood, other bodily fluids, and in various tissues such as lymph nodes and spleen. CD14 is weakly expressed on subpopulations of human neutrophils and myeloid dendritic cells. |
| Cross-reactivity |
Clone TÜK4 is tested to recognize monocytes from
- Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
The antibody has also been reported to cross-react with pigtail monkey (Macaca nemestrina) and cotton-top tamarin (Sanguinus oedipus oedipus) monocytes1, as well as with pig, cow, sheep, goat, dog, mink, and rabbit cells2. |
| Clone | Isotype |
| TÜK4 | Mouse IgG2a |
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| Figure 1 |
| Human PBMCs were stained with CD14 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue (D), PerCP (E), PE-Vio770 (F), APC-Vio770 (G) or VioGreen (H) and analyzed by flow cytometry. |
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| B |
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| D |
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| E |
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| F |
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| G |
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| H |
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| Figure 2 |
| Rhesus monkey PBMCs were stained with CD14-FITC and analyzed by flow cytometry. |
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| Figure 3 |
| Cynomolgus monkey PBMCs were stained with CD14-APC and analyzed by flow cytometry. |
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