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| Overview |
| The CD8 antibody can be used to identify and enumerate human and non-human primate CD8+ cells by flow cytometry or fluorescence microscopy. The CD8 antibody recognizes the α-subunit of the antigen. |
| Details |
Background information
The CD8 antibody recognizes the human CD8 antigen which is strongly expressed on human cytotoxic T cells and thymocytes, and is also expressed on a subset of NK cells. The CD8 antigen is a disulfide-linked dimer that exists either as a CD8α homodimer or as a CD8α/β heterodimer. CD8 acts as a coreceptor for the T cell receptor and binds to the MHC Class I molecule. |
| Cross-reactivity |
The antibody is tested to cross-react with - Rhesus monkey (Macaca mulatta)
- Cynomolgus monkey (Macaca fascicularis)
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| Clone | Isotype |
| BW135/80 | Mouse IgG2a |
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| Figure 1 |
| Human PBMCs were stained with CD8 antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue® (D), VioGreen (E), PerCP (F), PE-Vio770 (G) or APC-Vio770 (H) and analyzed by flow cytometry. Cells were gated on viable lymphocytes. |
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| B |
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| D |
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| E |
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| G |
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| H |
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| Figure 2 |
| Rhesus monkey peripheral blood lymphocytes were stained with CD8-FITC and analyzed by flow cytometry. |
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| Figure 3 |
| Cynomolgus monkey PBMCs were stained with CD8-APC and analyzed by flow cytometry. |
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