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| Overview |
| The CD3 antibody can be used to identify and enumerate human CD3+ cells by flow cytometry or fluorescence microscopy. The CD3 antibody recognizes all T cells, i.e. it reacts with 70–80% of human peripheral blood lymphocytes and with 65–85% of all thymocytes. |
| Details |
Background information
The CD3 antibody recognizes the human CD3 antigen which is present on mature human T cells, thymocytes, and a subset of NK cells. CD3 is associated with the T cell receptor (TCR) and is responsible for its signal transduction. The CD3 antigen is a complex of five invariable chains: γ, δ, ε, ζ, and η. The epitope recognized by the antibody is located on the ε-chain of the CD3 complex. |
| Clone | Isotype |
| BW264/56 | Mouse IgG2a |
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| Figure 1 |
| Human peripheral blood lymphocytes were stained with CD3 antibodies conjugated to FITC (A), PE (B), APC(C), VioBlue (D), VioGreen (E), PerCP (F), PE-Vio770 (G), or APC-Vio770 (H) and analyzed by flow cytometry. |
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| B |
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| D |
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| E |
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| F |
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| G |
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| H |
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