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 - Reliable results: highly standardized experimental procedure
- Versatile: enumeration in whole blood or PBMCs, even from small samples
- Convenient: everything you need in one box
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| Overview |
The Blood Dendritic Cell Enumeration Kit was developed for easy identification and enumeration of dendritic cells (DCs) and DC subsets in whole blood or PBMCs by flow cytometry. All reagents required for the analysis are included in the kit.
Applications of the kit include monitoring of peripheral blood DC frequency and number, for example, after hematopoietic stem cell mobilization3, or in the course of pathogenic infections, for example by HIV.4 Furthermore, DC numbers were determined in patients with chronic graft versus host disease5, inflammatory bowel disease6, psoriatic arthritis and rheumatoid arthritis10, under long-term immunosuppression8, lymphoproliferative disease of granular lymphocytes9, or in patients with hepatocellular carcinoma, in order to analyze the immunosuppressive role of IL-10 on circulating dendritic cells7. |
| Details |
Background information
The kit is based on the dendritic cell-specific surface antigens CD303 (BDCA-2), CD141 (BDCA-3), and CD1c (BDCA-1).1 The markers allow easy identification of three distinct dendritic cell subsets in blood: plasmacytoid dendritic cells (PDCs) are identified by CD303 (BDCA-2), type-1 myeloid dendritic cells (MDC1s) are identified by CD1c (BDCA-1), and type-2 myeloid dendritic cells (MDC2s) are identified by CD141 (BDCA-3) expression. The distinct dendritic cell subsets are analyzed within one sample by three-color (PDCs and MDC1s) or four-color (PDCs, MDC1s and MDC2s) flow cytometry.
The kit includes all reagents needed for a complete analysis: pre-mixed antibody cocktails for dendritic cell detection and for isotype control, a dead cell discrimination dye, and ready-to-use reagents for erythrocyte lysis and cell fixation. The standardized reagents and protocol ensure reproducible results.2 The dead cell dye used in this kit allows staining of dead cells in combination with cell fixation. This is particularly useful when working with hazardous samples.
The assay requires only small samples (either 300 µL of whole blood or 1×106 PBMCs, both for sample and control). |
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| Figure 1 |
| Dendritic cell subset–specific antigens. |
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| Figure 2 |
| Staining procedure of the Blood Dendritic Cell Enumeration Kit. |
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| Products |
| Blood Dendritic Cell Enumeration Kit, human |
- for 50 tests (1)
Download datasheet
130-091-086
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- for 2×50 tests (1)
Download datasheet
130-091-324
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| (1) One test corresponds to fluorescent labeling of 2×300 μL whole blood or 2×106 PBMCs. |
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| References |
| 1. Dzionek et al. (2000) J. Immunol. 165: 6037–6046. |
| 2. Dzionek et al. (2002) Human Immunol. 36: 1133–1148. |
| 3. Arpinati et al. (2002) Bone Marrow Transplant. 29: 887–891. |
| 4. Chehimi et al. (2002) J. Immunol. 168: 4769–4801. |
| 5. Clark et al. (2003) Transplant. 75: 221–225. |
| 6. Baumgart et al. (2005) Gut 54: 228–236. |
| 7. Beckebaum et al. (2004) Clin. Cancer Res. 10: 7260–7269. |
| 8. Hackstein et al. (2005) J.Transplant. 5: 2945–2953. |
| 9. Zambello et al. (2005) Blood 106: 3926–3931. |
| 10. Jongbloed et al. (2006) Arthritis Research & Therapy 8: R15 |
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