CD304 (BDCA-4/Neuropilin-1) ( #130-090-533 #130-090-900 #130-090-748 #130-090-693 )

CD304 (BDCA-4/Neuropilin-1)

Overview

This antibody allows the direct identification of CD304 (BDCA-4/Neuropilin-1)⁺ plasmacytoid dendritic cells in blood and bone marrow by immunofluorescent staining and flow cytometric analysis.^1,4 The antibody is suitable for staining of fresh or formaldehyde-fixed cells in suspension. Staining with CD304 (BDCA-4) antibodies is recommended for flow cytometric evaluation if plasmacytoid dendritic cells are isolated using the CD303 (BDCA-2) MicroBead Kit.

Details

Background information
Clone AD5-17F6 recognizes the human CD304 (BDCA-4/Neuropilin-1) antigen. Like CD303 (BDCA-2), the CD304 (BDCA-4/Neuropilin-1) antigen¹ is specifically expressed on human plasmacytoid dendritic cells in blood^1–3 and bone marrow⁴. Unlike CD303 (BDCA-2), expression of CD304 (BDCA-4/Neuropilin-1) is up-regulated on plasmacytoid dendritic cells and induced on myeloid cells upon culturing.⁴ In inflamed tonsils, CD304 (BDCA-4/Neuropilin-1) expression, apart from plasmacytoid dendritic cells, is also detected primarily on follicular B helper memory T cells.⁴ CD304 (BDCA-4) is identical to neuropilin-1 (NP-1),⁴ a neuronal receptor, which is known to be expressed on numerous nonhematopoietic cell types, including neurons, endothelial, and tumor cells. CD1a⁺ dendritic cells generated ex vivo from monocytes or hematopoietic precursor cells are CD304 (BDCA-4/Neuropilin-1)⁺ but CD303 (BDCA-2)^–.¹

Clone	Isotype
AD5-17F6	Mouse IgG1

Figure 1

Peripheral blood leukocytes were stained with CD304 (BDCA-4/Neuropilin-1)-PE and CD123-APC. Note that CD304 (BDCA-4) is exclusively expressed on plasmacytoid dendritic cells.