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| Overview |
| The antibody is suitable for the identification and enumeration of villous M cells by flow cytometry or fluorescence microscopy as well as for immunohistochemistry (paraffin-embedded or frozen sections).1 |
| Details |
Background information
Microfold cells or membranous cells (M cells) have a high transcytotic activity and play an important role in the uptake of luminal antigens for induction of antigen-specific immune responses. This antibody (clone NKM 16-2-4) specifically binds to all subsets of M cells, but not epithelial cells or goblet cells located in Peyer's patches, nasopharynx-associated lymphoid tissue, and the intestinal villi in the gastrointestinal or respiratory tracts. |
| Clone | Isotype |
| NKM 16-2-4 | Rat IgG2c |
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| Figure 1 |
Mouse Peyer's patches were stained with Anti-Microfold (M) Cell antibodies conjugated to PE (A) or APC (B) as well as with CD45‑FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were
excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| B |
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| Products |
| Anti-Microfold (M) Cell-PE, mouse |
- for 100 tests (1)
Download datasheet
130-096-155
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| Anti-Microfold (M) Cell-APC, mouse |
- for 100 tests (1)
Download datasheet
130-096-153
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| Anti-Microfold (M) Cell pure, mouse |
- 100 µg in 1 mL
Download datasheet
130-096-148
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Nochi, T. et al. (2007) J. Exp. Med. 204: 2789–2796. |
| 2. Terahara, K. et al. (2008) J. Immunol. 180: 7840–846. |
| 3. Kim, S-H. et al. (2010) J. Immunol. 185: 5787–5795. |
| 4. Kim, D-Y. et al. (2011) J. Immunol. 186: 4253–426. |
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