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| Overview |
The antibody is suitable for the identification and enumeration of CD115+ cells by flow cytometry or fluorescence microscopy.
CD115 is expressed on mouse monocytes, macrophages, and osteoclasts, as well as on common dendritic cell precursors and macrophage/dendritic cell precursors. |
| Details |
Background information
Clone AFS98 recognizes the protein tyrosine kinase transmembrane receptor for macrophage colony-stimulating factor (M-CSF), also known as CSF-1 and interleukin 34 (IL-34). CD115 is a single-pass type I membrane protein. Receptor activation induces homo-dimerization, which leads to phosphorylation and ubiquitinylation of intracellular residues.
Applications
- CD115 has been proposed as a marker for the standardized identification and analysis of mouse monocytes.1
- Differentiation studies of monocytes and precursors.
- MACS Control experiments in combination with the CD115 MicroBead Kit, mouse.
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| Clone | Isotype |
| AFS98 | Rat IgG2a |
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| Mouse bone marrow cells were stained with Anti-CD115 antibodies conjugated to PE (A), APC (B), and Biotin (C), as well as with Anti-Ly-6C-PE or Anti-Ly-6C-APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD115-Biotin (C) were stained with Anti-Biotin-PE as well as Anti-Ly-6C-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| Products |
| CD115-PE, mouse |
- for 100 tests (1)
Download datasheet
130-096-308
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| CD115-APC, mouse |
- for 100 tests (1)
Download datasheet
130-096-310
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| CD115-Biotin, mouse |
- for 100 tests (1)
Download datasheet
130-096-323
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| (1) One test corresponds to labeling of up to 107 cells in a total volume of 100 µL. |
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| References |
| 1. Breslin et al. (2011) J. Immunol. Meth.: Epub ahead of print, Apr. 2. |
| 2. Sudo et al. (1995) Oncogene 11: 2469–2476. |
| 3. Murayama et al. (1999) Circulation 99: 1740–1746. |
| 4. Sunderkotter et al. (2004) J. Immunol. 172: 4410–4417. |
| 5. Auffray et al. (2009) J. Exp. Med. 206: 595–606. |
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