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| Overview |
| The CD24 antibody is optimized for the detection of CD24 expressing cells from single-cell suspensions from solid tissue or cell lines by flow cytometry or fluorescent microscopy. The CD24 antibody can be used, for example, to differentiate CD44+CD24- breast cancer stem cells from CD24+ expressing cells from a primary tumor sample. |
| Details |
Background information
The human CD24 antigen is also known as heat-stable antigen (HSA). CD24 has been identified to be a negative marker for breast cancer stem cells1 and a positive marker for ovarian2 or pancreatic cancer stem cells.3 |
| Clone | Isotype |
| 32D12 | Mouse IgG1 |
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| Figure 1 |
| CD24+ cells from peripheral blood were stained with CD24 antibodies conjugated to FITC (A) or PE (B) or APC (C) and analyzed by flow cytometry using the MACSQuant® Analyzer. CD24+ cells from the CML cell line K562 were stained with CD24-PE (D) and analyzed by flow cytometry using the MACSQuant® Analyzer. As seen in the histogram the expression levels vary from CD24low to CD24high. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. |
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| B |
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| Products |
| CD24-FITC, human |
- for 100 tests (1)
Download datasheet
130-095-952
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| CD24-PE, human |
- for 100 tests (1)
Download datasheet
130-095-953
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| CD24-APC, human |
- for 100 tests (1)
Download datasheet
130-095-954
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| (1) One test corresponds to fluorescent labeling of up to 107 cells in a total volume of 100 μL. |
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| References |
| 1. Al-Hajj et al. (2003) Proc Natl Acad Sci 100: 3983-3988 |
| 2. Gao et al. (2010) Oncogene 29: 2672-2680 |
| 3. Li et al. (2007) Cancer Res 67 (3): 1030-1037 |
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