CD127 ( #130-094-888 #130-094-889 #130-094-890 #130-094-891 #130-094-942 )

CD127

Overview

The antibody is suitable for the identification and enumeration of human and non-human primate CD127+ cells, e.g. for the analysis of human regulatory T cells by using, for example, CD4-FITC, CD25-PE, and CD127-APC monoclonal antibodies. Moreover, it can be used for the identification of memory CD8⁺ T cell precursors within an effector T cell population.

Details

Background information
Clone MB15-18C9 recognizes the CD127 antigen, which is the α-chain of the IL-7 receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc).¹ CD127 can be identified on immature B cells through the early pre-B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation.^2,3 On regulatory T cells CD127 is absent⁴ and its expression is inversely correlated with FoxP3 expression and suppressive function.^5,6 CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.¹

Cross-reactivity

This antibody was shown to react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Clone	Isotype
MB15-18C9	Mouse IgG2a

Figure 1

Human PBMCs were stained with CD127 antibodies conjugated to FITC (A), PE (B), or APC (C), as well as with CD4-PE or CD4‑APC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cells labeled with CD127-Biotin (D) were stained with Anti‑Biotin-PE and CD4-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Figure 2

Non-human primate PBMCs from Rhesus monkey (A, B: chinese origin; C, D: indian origin) were stained with CD127 antibodies conjugated to PE (A, C), or APC (B, D), as well as with CD4-FITC and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.