Anti-HLA-DR ( #130-095-295 #130-095-293 #130-095-298 #130-095-297 #130-095-291 )

Anti-HLA-DR

Overview

The antibody is suitable for the identification and enumeration of human and non-human primate HLA-DR⁺ cells by flow cytometry or fluorescence microscopy. Suitable applications are for example

Detection of antigen-presenting cells from PBMCs, cord blood, bone marrow, and tissues
Study of expression level of HLA-DR on professional APC-activated T cells or epithelial cells
Evaluation of MACS® Separations

Details

Background information
Anti-HLA-DR antibodies react with the human major histocompatibility (MHC) class II antigen HLA-DR. HLA-DR is constitutively expressed on professional antigen-presenting cells like dendritic cells, B cells, and monocytes/macrophages. Its expression is further up-regulated upon activation. On T cells, NK cells, hematopoietic precursor cells, and some epithelial cells the expression of HLA-DR is induced by cell activation.

Cross-reactivity

Clone AC122 is tested to cross-react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Cross-reactivity is reported with cells from
African green monkey (Chlorocebus aethiops)

Common marmoset (Callithrix jacchus)
Guinea pig

Clone	Isotype
AC122	Mouse IgG2a

Figure 1

Human PBMCs were stained with Anti-HLA-DR antibodies conjugated to FITC (A), PE (B), APC (C), VioBlue® (D), or PerCP (E) as well as with CD14-PE or -APC and analyzed by flow cytometry using the MACSQuant® Analyzer.

Figure 2

Rhesus monkey (A) or cynomolgus monkey (B) PBMCs were stained with Anti-HLA-DR-APC as well as with CD123-PE (clone 7G3). Cells were analyzed by flow cytometry using the MACSQuant® Analyzer.