CD90.1 ( #130-094-526 #130-094-524 #130-094-588 #130-094-527 #130-094-528 #130-094-525 )

CD90.1

Overview

The CD90.1 antibody is suitable to identify and enumerate mouse or rat CD90⁺ cells by flow cytometry or fluorescence microscopy.

Details

Background information
The mouse monoclonal antibody His51 reacts with the rat CD90 (Thy-1) and mouse CD90.1 (Thy1.1) antigens, which belong to a GPI-anchored conserved membrane glycoprotein. In the mouse strains AKR/J, PL, and FVB/N, CD90.1 is a pan T cell marker and can be found on thymocytes, hematopoietic stem cells in the bone marrow, intra-epithelial cells (dendritic epidermal cells) in skin, and on neurons, such as retinal ganglion cells.
In the rat, the CD90 antigen is expressed on thymocytes, recent thymic emigrants, hematopoietic stem cells, on neurons such as retinal ganglion cells, and on other cell types. The antibody does not cross-react with CD90.2 (Thy1.2).

Cross-reactivity

Reacts with rat CD90 (Thy-1) and mouse CD90.1 (Thy1.1)

Clone	Isotype
His51	Mouse IgG2a

Figure 1

Mouse spleen cells were stained with CD90.1 antibodies conjugated to FITC (A), PE (B), APC (C), or VioBlue™ (D) as well as with CD45R (B220)-PE or CD45R (B220)-APC and analyzed using the MACSQuant® Analyzer. Cells labeled with CD90.1-Biotin (E) were stained with Anti-Biotin-PE as well as CD45R (B220)-APC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.