Anti-IL-17A ( #130-094-520 #130-094-521 #130-094-519 #130-096-656 #130-096-748 )

Anti-IL-17A

Overview

The antibody is suitable for the identification and enumeration of IL-17A–producing cells or antigen-specific T cells upon restimulation with the respective antigen by flow cytometry or fluorescence microscopy. It can be used with human or non-human primate cells. Further applications of the antibody are analyses of cytokine production by rare cells through in-column intracellular staining technology in combination with MACS® Separations.

Details

Background information
Clone CZ8-23G1 detects human interleukin 17. IL-17 (IL-17A, CTLA8) is produced by Th17 cells, a T helper subset distinct from Th1 and Th2 cells. These cells also secrete IL-17F and IL-22 and express the NK cell marker CD161¹. IL-17 secretion has also been described for other cell types, for example, CD8⁺ memory T cells.
Th17 cells are involved in the recruitment of neutrophils to control early stages of infections by a number of pathogens, such as extracellular bacteria and fungi. IL-17 and Th17 cells play an important role in immune-mediated inflammatory diseases, such as rheumatoid arthritis, psoriasis, multiple sclerosis, asthma, and inflammatory bowel disease.

Cross-reactivity

The antibody has been tested to react with

Rhesus monkey (Macaca mulatta)
Cynomolgus monkey (Macaca fascicularis)

Clone	Isotype
CZ8-23G1	Mouse IgG1

Figure 1

Human PBMCs were incubated with or without CytoStim for 6 hours. After 2 hours brefeldin A was added. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-17 antibodies conjugated to FITC (A), PE (B), APC (C), PE-Vio770 (D) or APC-Vio770 (E). Cell surface staining was performed with CD4-FITC or CD4-PE and CD154-PE or CD154-APC. Cells were analyzed using the MACSQuant® Analyzer. Gating was performed according to CD4 expression and side scatter properties of the cells. Cell debris was excluded from the analysis in a propidium iodide versus Anti-IL-17-PE dot plot.

CytoStim-stimulated PBMCs

Unstimulated PBMCs

Figure 2

PBMCs from cynomolgus monkey were incubated with CytoStim for 6 hours or left untreated. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-17A-PE and CD154-APC. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer.

A: CytoStim-stimulated PBMCs

B: Unstimulated PBMCs

Figure 3

PBMCs from rhesus monkey were incubated with CytoStim for 6 hours or left untreated. After two hours, brefeldin A was added. The cells were fixed, permeabilized, and intracellularly stained with Anti-IL-17A-PE and CD154-APC. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer.

A: CytoStim-stimulated PBMCs

B: Unstimulated PBMCs