Anti-IL-17A ( #130-094-298 #130-094-296 #130-094-294 )

Anti-IL-17A

Overview

This antibody has been developed for intracellular staining of IL-17A in fixed and permeabilized cells for flow cytometric analysis. Cells can be stained in suspension or by in-column intracellular staining technology in combination with MACS® Separation. A functional grade antibody for neutralization of IL-17 activity is also available.

Details

Background information
The Anti-IL-17A antibodies are designed for intracellular staining of IL-17A–producing cells.
Interleukin 17 (IL-17) is a family of cytokines that play a central role in adaptive immunity as well as autoinflammatory disorders.¹ Data from several mouse models suggest that IL-17 plays a key role in the host defense against certain extracellular bacterial infections.
Recently, a new mouse T helper cell subset has been identified, which is dinstinct from Th1 and Th2 cells: IL-17–producing CD4⁺ T helper (Th17) cells. Th17 cells preferentially produce IL-17A, IL-17F, IL-21, and IL-22. Receptors for IL-17 and IL-22 are expressed on various epithelial tissues, thus Th17 cells are crucial for the cross-talk between immune system and tissues.² It is now established that Th17 cells are responsible for driving autoimmune inflammation.
After stimulation of mouse T cells, IL-17 is not only secreted by CD4⁺ T cells, but also by CD8⁺ T cells and γδ T cells.

Clone	Isotype
TC11-18H10	Rat IgG1

Figure 1

Mouse spleen cells were incubated with or without PMA/ionomycin for 6 hours. After 2 hours brefeldin A was added. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-17-PE. Cell surface staining was performed with CD4-APC. Cells were analyzed by flow cytometry. B cells and cell debris were excluded from the analysis.

a: Stimulated sample

b: Unstimulated control