Treg Detection Kits (CD4/CD25/FoxP3), mouse ( #130-094-165 #130-094-164 )

Treg Detection Kits (CD4/CD25/FoxP3), mouse

Overview

The Treg Detection Kits (CD4/CD25/FoxP3), mouse, have been developed for the convenient detection of CD4⁺CD25⁺FoxP3⁺ regulatory T (Treg) cells. The kits include all required reagents for the analysis of mouse Treg cells by flow cytometry.

Details

Background information
Treg cells are a subset of T cells with the ability to suppress harmful immunological reactions to self and foreign antigens. Due to the lack of a specific marker for Treg cells, a combination of CD4, CD25, and FoxP3 is commonly used for their identification. The Treg Detection Kit (CD4/CD25/FoxP3) includes all reagents necessary for cell surface staining of CD4 and CD25, and intracellular staining of FoxP3 with a special FoxP3 Staining Buffer Set. The kit also includes an optimized protocol.

Clone	Isotype
Anti-FoxP3	Mouse IgG1
CD4	Rat IgG2b
CD25	Rat IgM

Figure 1

Mouse splenocytes were stained using the Treg Detection Kit (CD4/CD25/FoxP3) (PE) and analyzed by flow cytometry. For the dot plot that shows CD25-APC and Anti-FoxP3-PE staining, gating was performed according to CD4-FITC staining.

Figure 2

Mouse splenocytes were stained using the Treg Detection Kit (CD4/CD25/FoxP3) (APC) and analyzed by flow cytometry. For the dot plot that shows CD25-PE and Anti-FoxP3-APC staining, gating was performed according to CD4-FITC staining.