Mouse IL-17 Secretion Assay – Detection Kits ( #130-094-205 #130-094-207 )

Mouse IL-17 Secretion Assay – Detection Kits

Overview

The IL-17 Secretion Assay was developed for the sensitive detection of mouse IL-17A–secreting cells.

Details

Background information
Interleukin 17 (IL-17) is a family of cytokines that play a central role in adaptive immunity as well as autoinflammatory disorders.¹ Data from several mouse models suggest that IL-17 plays a key role in the host defense against certain extracellular bacterial infections.
Recently, a new mouse T helper cell subset has been identified, which is dinstinct from Th1 and Th2 cells: IL-17–producing CD4⁺ T helper (Th17) cells. Th17 cells preferentially produce IL-17A, IL-17F, IL-21, and IL-22. Receptors for IL-17 and IL-22 are expressed on various epithelial tissues, thus Th17 cells are crucial for the cross-talk between immune system and tissues.² It is now established that Th17 cells are responsible for driving autoimmune inflammation.
After stimulation of mouse cells, IL-17 is not only secreted by CD4⁺ T cells, but also by CD8⁺ T cells, γδ T cells, NK cells, and granulocytes.

Mouse splenocytes were stimulated for 3 hours with ionomycin (1 μg/mL) and PMA (10 ng/mL) or left untreated. Cells were stained by using the Mouse IL-17 Secretion Assay – Detection Kit (PE). T helper cells were counterstained by using CD4-APC. B cells and dead cells were excluded from the analysis.

Stimulated sample

Unstimulated control