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Stem cell research is one of the fastest growing fields in biological sciences. Flow cytometry has played a significant role in supporting recent developments, such as in the characterization of stem cells and their downstream progeny.
The MACSQuant Analyzer assays for stem cell biology include:
- Characterization and enumeration of HSCs
- Characerization of MSCs
- Identification and characterization of iPS and ES cells
- Identification and characterization of Cancer Stem Cells
- Cell fate tracking with fluorescent proteins
- Monitoring cellular viability, apoptosis and cell cycle
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References
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| Albanese, P et al.Glycosaminoglycan mimetics – induced mobilization of hematopoietic progenitors and stem cells in mouse peripheral blood: Structure/function insights (2009) Exp Hematol. |
| Chaudhuri, AA et al. Oncomir miR-125b regulates hematopoiesis by targeting the gene Lin28A (2012) Proc Natl Acad Sci U S A. |
| Chow, KS et al. Isolation and characterization of Hoeschst(low) CD45(negative) mouse lung mesenchymal stem cells (2011) J of Vis Exp. (JOVE). |
| De Bruyn, C et al. A rapid, simple and reproducible method for the isolation of mesenchymal stromal cells from Wharton’s jelly without enzymatic treatment (2010) Stem Cells and Development. |
| Fellenberg, J et al. A microRNA signature differentiates between giant tumor derived neoplastic stromal cells and mesenchymal stem cells (2012) Cancer Lett. |
| Frescaline, G et al. Glycosaminoglycans mimetics potentiate the clonogenicity, proliferation, migration and differentiation properties of rat mesenchymal stem cells (2012) Stem Cell Res. |
| Jun, DH et al. The pathology of bleomycin induced fibrosis is associated with loss of resident lung mesenchymal stem cells which regulate effector T-cell proliferation (2011) Stem Cells. |
| Lagadec, C et al. Radiation-induced reprogramming of breast cancer cells (2012) Stem Cells. |
| Lagadec, C et al. Oxygen levels do not determine radiation survival of breast cancer stem cells (2012) PLoS One. |
| Najar, M et al. Mesenchymal stromal cells promote or suppress the proliferation of T lymphocytes from cord blood and peripheral blood: the importance of low cell ratio and role of interleukin-6 (2009) Cytotherapy. |
| Niwa, A et al. A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors (2011) PLoS ONE. |
| Raicevic, G et al. The source of human mesenchymal stromal cells influences their TLR profile as well as their functional properties (2011) Cell Immunol. |
| Sandt, C et al. Identification of spectral modifications occuring during reprogramming of somatic cells (2012) PLoS One. |
| Van Linthout, S et al. Mesenchymal stem cells improve murine acute coxsackievirus B3-induced myocarditis (2011) Eur Heart J. |
| Vlashi, E et al. Metabolic state of glioma stem cells and nontumorigenic cells (2011) PNAS. |
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