CD146 (LSEC) MicroBeads
Description CD146 (LSEC) MicroBeads, formerly termed Anti-LSEC MicroBeads, were developed to isolate mouse liver sinusoidal endothelial cells (LSECs). The CD146 (LSEC) antibody binds to mouse endothelial cells, including liver sinusoidal cells, to smooth-muscle cells, and basal membrane.1 LSECs are microvascular endothelial cells lining the hepatic sinusoidal wall. Their strategic positioning favors a tight interaction with lymphocytes migrating through the liver. They possess a high capacity for antigen uptake and processing but express, in contrast to professional antigen-presenting cells (e.g. dendritic cells), only low levels of costimulatory molecules.2 LSECs are supposed to mainly contribute to the control of immune responses against circulating soluble antigens in the liver. Applications Mouse LSECs isolated with CD146 (LSEC) MicroBeads are suitable for functional studies and co-culture experiments. Columns For positive selection: MS, LS, XS, or autoMACSâ„¢ Columns; for depletion: LD, CS, D, or autoMACS Columns. Further information Preparation of single-cell suspensions from mouse liver with Collagenase D treatment [PDF; 65,2 KB]		Figure 1 LSECs were isolated from a mouse liver cell suspension using CD146 (LSEC) MicroBeads, an MS Column, and a MiniMACSâ„¢ Separator. The cells were stained with CD146 (LSEC)-FITC and CD49b(DX5)-APC. Isolated LSECs Mouse liver cells before separation Mouse liver cells depleted of LSECs