Anti-MHC Class II MicroBeads
Description Mouse Anti-MHC Class II MicroBeads are used for the positive selection or depletion of MHC class II+ cells from lymphoid and non-lymphoid tissue or from blood. MHC class II is expressed on B cells, macrophages, monocytes, dendritic cells, thymic epithelial cells, and on subsets of hematopoietic progenitor cells. The MicroBeads bind to MHC class II haplotypes I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek, which are expressed by the most common inbred mouse strains, e.g. C57BL/6, BALB/c, or 129/Sv. MHC II haplotypes I-Af, I-Ak, and I-As are not recognized. Applications Isolated MHC class II+ cells are suitable for studies on antigen presentation such as in T cell stimulation assays. Anti-MHC Class II MicroBeads were used for positive selection of Langerhans cells from epidermis1, for the isolation of untouched T cells from lymph nodes by depletion of MHC class II+ cells2, or in combination with other MicroBeads for isolation of untouched T cells from spleen3,4. Columns For positive selection: MS, LS, XS, or autoMACSâ„¢ Columns; for depletion: LD, CS, D, or autoMACS Columns.		Figure 1 Isolation of untouched T cells from mouse lymph nodes by depletion of MHC class II expressing cells using Anti-MHC Class II MicroBeads, a MiniMACSâ„¢ Separator, and an MS Column. Lymph node cells before separation Enriched T cells after depletion of MHC class II+ cells